The correct order of occurrence of the important steps of decomposition of
complex organic matter is:
Fragmentation: Detritivores (e.g., earthworms) break down detritus into smaller particles.
Leaching: Water-soluble inorganic nutrients seep down into the soil horizon and get
precipitated as unavailable salts. Catabolism: Microbial enzymes degrade the simpler
soluble compounds into even simpler inorganic substances. Humification: This is the process
of formation of humus. Humus is a dark-colored amorphous substance that is highly resistant
to microbial action and undergoes decomposition at an extremely slow rate. It is colloidal in
nature and serves as a reservoir of nutrients. Mineralisation: The further degradation of
humus by some microbes releases inorganic nutrients (minerals) back into the soil. The
fourth step in the process is Humification. During humification, the partially decomposed
organic matter is transformed into a stable, dark-colored amorphous substance called humus.
Humus is rich in lignin and cellulose derivatives and is highly resistant to microbial action,
decomposing very slowly. It is colloidal in nature, meaning it has a large surface area for
nutrient retention, and it plays a crucial role in soil fertility by acting as a reservoir of
essential nutrients like nitrogen, phosphorus, and potassium. Humus also improves soil
structure, aeration, and water-holding capacity.
Correct Answer : The correct order is Fragmentation, Leaching, Catabolism,
Humification, Mineralisation. The fourth step, Humification, is the formation of humus, a
dark, stable, nutrient-rich substance resistant to rapid decomposition, contributing
significantly to soil fertility.