Question

Arrange the following steps of DNA fingerprinting in proper sequence:
(A) Hybridization using labeled VNTR probe
(B) Separation of DNA fragments by electrophoresis
(C) Digestion of DNA by restriction endonucleases
(D) Blotting of separated DNA fragments to nylon
(E) Isolation of DNA
Choose the correct answer from the options given below:

• A. (C), (A), (B), (D), (E)
• B. (E), (C), (B), (D), (A)
• C. (B), (C), (D), (E), (A)
• D. (C), (D), (B), (A), (E)

Answer 1

The Correct Option is B

The correct order for DNA fingerprinting is: (E) Isolation of DNA, (C) Digestion by restriction endonucleases, (B) Separation by electrophoresis, (D) Blotting to nylon membrane, and (A) Hybridization with a labeled probe. DNA fingerprinting is a powerful technique used for identification based on DNA variations.

Answer 2

The steps involved in DNA fingerprinting are as follows:

• Isolation of DNA (E): The first step is to extract the DNA from the sample (blood, hair, tissue, etc.).
• Digestion of DNA by restriction endonucleases (C): The DNA is then cut into smaller fragments using restriction enzymes.
• Separation of DNA fragments by electrophoresis (B): The DNA fragments are separated based on their size using agarose gel electrophoresis.
• Blotting of separated DNA fragments to nylon (D): The separated DNA fragments are transferred from the gel onto a nylon membrane for further analysis.
• Hybridization using labeled VNTR probe (A): A labeled probe (which binds to specific DNA regions) is used to detect the presence of specific DNA sequences, revealing the individual's unique DNA pattern.

Thus, the correct sequence is (E), (C), (B), (D), (A).