Question

Arrange the following in the correct sequence followed in Ultra Performance Liquid Chromatography.
A. Mobile phase reservoir
B. Guard column
C. Separation column
D. Detector

• A. A, B, C, D
• B. A, B, D, C
• C. B, A, D, C
• D. C, B, D, A

Hint:

Think of it like a train journey. The mobile phase is the train. It starts at the station (\textbf{Reservoir}), goes through a security check (\textbf{Guard Column}), travels the main route where passengers get off at different stops (\textbf{Separation Column}), and finally, the arrival of each passenger group is noted at the destination (\textbf{Detector}).

Answer 1

The Correct Option is A

The components of a chromatography system are arranged in the order of the fluid's path.
Step 1: The Source. The process starts with the solvent that will carry the sample.
A. Mobile phase reservoir: This holds the solvent (mobile phase) that is pumped through the system.

Step 2: Protection. Before the main separation, the expensive main column is protected.
B. Guard column: This is a small, disposable column placed before the main column to filter out impurities and particles that could damage the separation column.

Step 3: The Main Process. This is where the chemical separation of the sample components occurs.
C. Separation column (or analytical column): This is the heart of the system, where the components of the mixture are separated based on their interaction with the column's stationary phase.

Step 4: The Result. After separation, the components must be detected.
D. Detector: This device measures the components as they exit the column, producing a signal that is recorded as a chromatogram.
The correct sequence for the flow path is A \(\rightarrow\) B \(\rightarrow\) C \(\rightarrow\) D.