Question

A thermostable DNA polymerase is isolated from:

• A. Thermus aquaticus
• B. Agrobacterium tumefaciens
• C. E. coli
• D. Salmonella typhimurium

Answer 1

The Correct Option is A

The thermostable DNA polymerase, known as Taq polymerase, is isolated from Thermus aquaticus, a bacterium found in hot springs. Taq polymerase remains stable at high temperatures, making it ideal for use in Polymerase Chain Reaction (PCR) techniques where repeated cycles of heating are needed to denature DNA. This enzyme’s discovery revolutionized molecular biology by enabling rapid DNA amplification.

Answer 2

A thermostable DNA polymerase is an enzyme that can withstand high temperatures, making it ideal for processes that involve heat, such as the Polymerase Chain Reaction (PCR). The thermostable DNA polymerase commonly used in PCR is Taq polymerase, which is isolated from the bacterium Thermus aquaticus. This bacterium thrives in high-temperature environments, such as hot springs, which is why its DNA polymerase is particularly stable at the high temperatures used in PCR.