The question centers around the concept of speciation, which is a fundamental concept in evolutionary biology. Here, we are examining a situation where a population of fish has been divided into two separate groups due to geographical isolation—the lake drying up into two separate bodies of water. Over time, these groups have diverged enough that they can no longer interbreed, meaning they have become distinct species.
The correct answer is species because the inability to mate and produce viable offspring signifies speciation. This process results in the development of distinct species under the principle of reproductive isolation.
To clarify why other options are incorrect:
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 