The work done is calculated as:
\( W = F \cdot d \cdot \cos\theta \),
where:
The force required to move the box at constant speed is equal to the weight of the box since friction is negligible:
\( F = m \cdot g = 20 \text{ kg} \cdot 9.8 \text{ m/s}^2 = 196 \text{ N}. \)
The work done is:
\( W = 196 \cdot 20 \cdot \cos 45^\circ = 196 \cdot 20 \cdot \frac{1}{\sqrt{2}} \approx 2790 \text{ J}. \)
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer)
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
Which of the following is/are CORRECT for the two molecules shown?