Question:
Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?
Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?
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Insertional inactivation reduces dependency on antibiotics and provides a safer alternative for identifying recombinant colonies.
Updated On: Jan 18, 2025
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Solution and Explanation
Insertional inactivation ensures that the cloned DNA disrupts the marker gene, providing a visual or functional confirmation of successful transformation (e.g., loss of antibiotic resistance or colour change).
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