Question:
Which vector can clone only a small fragment of DNA?
Which vector can clone only a small fragment of DNA?
Updated On: Jul 12, 2022
- Bacterial artificial chromosome
- Yeast artificial chromosome
- Plasmid
- Cosmid
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The Correct Option is C
Solution and Explanation
Plasmids are extra chromosomal, self replicating usually circular double stranded DNA molecules found naturally in many bacteria and also in some yeast. These plasmids have been modified to be used as vectors. They can clone DNA fragments of about 10 kbp size while cosmid can carry upto 45 kbp, YAC can carry upto 1000-2500 kbp and BAC can carry around 300 - 350 Kbp long DNA fragments.
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.