Question:
Which one of the following statements is INCORRECT about hybridoma production?
Which one of the following statements is INCORRECT about hybridoma production?
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Hybridomas always produce monoclonal antibodies—identical antibodies from a single B-cell clone.
Updated On: Jan 2, 2026
- Hybridoma cells can use hypoxanthine and thymidine
- DNA synthesis in myeloma cells is blocked by aminopterin
- Hybridoma cells are made to produce polyclonal antibodies
- Polyethylene glycol is used to fuse myeloma cells to B-cells
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The Correct Option is C
Solution and Explanation
Hybridoma technology is used to produce monoclonal antibodies, not polyclonal antibodies.
(A) is correct because hybridoma cells survive in HAT medium by using hypoxanthine and thymidine.
(B) is correct because aminopterin blocks de novo DNA synthesis in myeloma cells.
(D) is correct since PEG is used for cell fusion.
Therefore, the only incorrect statement is that hybridomas produce polyclonal antibodies.
(A) is correct because hybridoma cells survive in HAT medium by using hypoxanthine and thymidine.
(B) is correct because aminopterin blocks de novo DNA synthesis in myeloma cells.
(D) is correct since PEG is used for cell fusion.
Therefore, the only incorrect statement is that hybridomas produce polyclonal antibodies.
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