Question

Which one of the following is NOT a tool in recombinant DNA technology?

• A. Restriction endonuclease
• B. Prokaryotic nucleoid
• C. Vector
• D. Reverse transcription

Hint:

Recombinant DNA technology involves enzymes like restriction endonucleases, vectors for DNA insertion, and reverse transcription for synthesizing DNA from RNA.

Answer 1

The Correct Option is B

Step 1: Understanding recombinant DNA technology tools.
Recombinant DNA technology involves various tools like restriction endonucleases, vectors, and enzymes involved in reverse transcription to manipulate DNA. The prokaryotic nucleoid, however, is not a tool used in the process. It is a region in prokaryotic cells where DNA is located.
Step 2: Analyzing the options.
(A) Restriction endonuclease: Incorrect — Restriction endonucleases are essential tools in recombinant DNA technology as they cut DNA at specific sites.
(B) Prokaryotic nucleoid: Correct — The prokaryotic nucleoid is not a tool in recombinant DNA technology; it refers to the region in prokaryotic cells where the genetic material is found.
(C) Vector: Incorrect — Vectors are carriers used to transfer foreign genetic material into host cells.
(D) Reverse transcription: Incorrect — Reverse transcription is the process of synthesizing DNA from RNA, which is used in recombinant DNA technology.
Step 3: Conclusion.
The correct answer is (B) Prokaryotic nucleoid, as it is not a tool used in recombinant DNA technology.