The breakdown of starch involves various enzymes that act on the starch polysaccharide to release sugars. The enzymes that break down starch include:
- \( \alpha \)-Amylase: This enzyme breaks starch into maltose and dextrins by cleaving the internal bonds of the starch molecule.
- \( \beta \)-Amylase: This enzyme sequentially releases maltose units from the non-reducing ends of the starch molecule. This process involves the removal of two glucose units at a time, producing maltose.
- Glucoamylase: This enzyme hydrolyzes starch and maltose to produce glucose units, but it is not involved in releasing maltose from starch.
- Pullulanase: This enzyme acts specifically on pullulan (a polysaccharide) and breaks down α-1,6-glucosidic linkages but does not play a role in the breakdown of starch into maltose.
Thus, the enzyme that sequentially releases maltose from starch is \( \beta \)-Amylase.
Final Answer:
\[
\boxed{\beta \text{-Amylase}}.
\]