Question:
Which of the statements is correct in the context of observing DNA separated by agarose gel electrophoresis ?
Which of the statements is correct in the context of observing DNA separated by agarose gel electrophoresis ?
Updated On: Jul 5, 2022
- DNA can be seen in visible light
- DNA can be seen without staining in visible light
- Ethidium bromide stained DNA can be seen in visible light
- Ethidium bromide stained DNA can be seen under exposure to UV light
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The Correct Option is D
Solution and Explanation
DNA cannot be seen in visible light, so options a and b are incorrect. Staining by ethidium bromide and presence of UV light are necessary to see DNA.
So, the correct answer is 'Ethidium bromide stained DNA can be seen under exposure to UV light.'
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.