Question:
Which of the following tools of recombinant $DNA$ technology is incorrectly paired with its use?
Which of the following tools of recombinant $DNA$ technology is incorrectly paired with its use?
Updated On: Jul 7, 2022
- EcoRI - Production of sticky ends
- $DNA$ ligase - Multiplication of $rDNA$ molecules
- ori- copy number
- Selectable marker - Identification of transformants
Hide Solution
Verified By Collegedunia
The Correct Option is B
Solution and Explanation
$DNA$ ligases are also called genetic gum. They join two individual fragments of double stranded $DNA$ by forming phosphodiester bonds between them thus help in sealing of $DNA$ fragments. Therefore acts as molecular glue. The enzyme used most often is $T_4\, DNA$ ligase.
Was this answer helpful?
0
0
Top Questions on Biotechnology
- Student to attempt either option (A) or (B):
(A) Name the Indian crop variety for which, in 1997, an American company got patent rights through the US Patent and Trademark Office. This is considered a case of Biopiracy. Justify.
OR
(B) State two purposes for which the Indian Government has set up GEAC (Genetic Engineering Approval Committee).- CBSE CLASS XII - 2025
- Biology
- Biotechnology
- Why are transgenic animals so called?
- With the help of an example each, explain the role of transgenic animals in the following:
- [(i)] Vaccine safety
- [(ii)] Biological products
- CBSE CLASS XII - 2025
- Biology
- Biotechnology
- Student to attempt either option (A) or (B): (A) How is DNA isolated in purified form from a bacterial cell?
OR
(B) Explain how human functional insulin was produced by using recombinant DNA (rDNA) technology.- CBSE CLASS XII - 2025
- Biology
- Biotechnology
- Gene therapy can be used to treat an ADA deficient patient. Explain.
Is it a permanent cure? How can ADA be cured permanently?- CBSE CLASS XII - 2025
- Biology
- Biotechnology
- Assertion (A): A piece of DNA inserted into an alien organism generally does not replicate, if not inserted into a chromosome.
Reason (R): \textit{Chromosomes have specific sequences called ‘ori’ region, where DNA replication is initiated.- CBSE CLASS XII - 2025
- Biology
- Biotechnology
View More Questions
Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.