Question

Which of the following methods is used to inject recombinant DNA into nucleus of animal cell directly?

• A. Transfection
• B. Transformation
• C. Gene gun
• D. Microinjection

Hint:

Microinjection is widely used in animal biotechnology for creating transgenic animals.

Answer 1

The Correct Option is D

Step 1: Methods of DNA introduction.
Different methods are used to introduce recombinant DNA into host cells — transformation, transfection, gene gun, and microinjection.
Step 2: Direct introduction into nucleus.
Microinjection involves directly injecting recombinant DNA into the nucleus of an animal cell using fine needles under a microscope.
Step 3: Conclusion.
Therefore, the correct method is Microinjection.