To determine which option is not a physical method for controlling microorganisms, we examine each option:
- Radiation: This is a physical method. It involves using electromagnetic waves, such as UV or gamma rays, to destroy microorganisms.
- Filtration: This is a physical method. It removes microorganisms by passing a fluid through a filter with pores small enough to prevent their passage.
- Disinfection: This is not a physical method. Disinfection generally involves chemical agents to eliminate microorganisms on surfaces.
- Desiccation: This is a physical method. It involves removing moisture to inhibit microbial growth and reproduction.
Based on the analysis, the correct answer is: Disinfection