Question:
Which of the following is not a desirable feature of a cloning vector?
Which of the following is not a desirable feature of a cloning vector?
Updated On: Apr 14, 2025
- Presence of origin of replication
- Presence of a marker gene
- Presence of single restriction enzyme site
- Presence of two or more recognition sites
Hide Solution
Verified By Collegedunia
The Correct Option is D
Solution and Explanation
In order to link the alien DNA, the cloning vector needs to have very few, preferably single recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
Was this answer helpful?
0
0
Top Questions on Biotechnology
- In recombinant DNA technology, which enzyme is used to cut DNA at specific recognition sites to produce restriction fragments?
- MHT CET - 2025
- Biology
- Biotechnology
- Combination of foreign DNA and vector DNA are together called as _______.
- MHT CET - 2025
- Biology
- Biotechnology
- In genetic engineering the Foreign DNA is called
- MHT CET - 2025
- Biology
- Biotechnology
- How many time needed for PCR?
- MHT CET - 2025
- Biology
- Biotechnology
- The picture given below shows:
(a) Roots of a typical control tobacco crop plant (infected).
(b) Transgenic tobacco plant showing healthy roots even after deliberate infection by nematode.
Explain how this transformation was achieved in the tobacco plant.- CBSE CLASS XII - 2024
- Biology
- Biotechnology
View More Questions
Questions Asked in NEET exam
- The magnetic moment of an iron bar is M. It is now bent in such a way that it forms an arc section of a circle subtending an angle of 60° at the centre. The magnetic moment of this arc section is
- NEET (UG) - 2024
- Magnetic moment
Identify the major product C formed in the following reaction sequence:
- NEET (UG) - 2024
- Chemical Reactions
- Given below are certain cations. Using inorganic qualitative analysis, arrange them in increasing group number from 0 to VI.
A) Al3+
B) Cu2+
C) Ba2+
D) Co2+
E) Mg2+
Choose the correct answer from the options given below- NEET (UG) - 2024
- Modern Periodic Law And The Present Form Of The Periodic Table
- The work done during reversible isothermal expansion of one mole of hydrogen gas at 25oC from a pressure of 20 atm to 10 atm is:
(Given R = 2.0 cal K-1 mol-1)- NEET (UG) - 2024
- Gas laws
- Consider the following reaction in a sealed vessel at equilibrium with concentrations of
N2 = 3.0 × 10–3 M, O2 = 4.2 × 10–3 M and NO = 2.8 × 10–3 M.
2NO(g)⇋N2(g) + O2(g)
If 0.1 mol L–1 of NO(g) is taken in a closed vessel, what will be degree of dissociation (a) of NO(g) at equilibrium?- NEET (UG) - 2024
- Equilibrium
View More Questions
Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.