Question

Which of the following is not a cloning vector for bacteria?

• A. Bacteriophage
• B. Plasmid
• C. pBR322
• D. T-DNA

Hint:

Remember: T-DNA is used for plant transformation via Agrobacterium, not bacterial cloning.

Answer 1

The Correct Option is D

Step 1: Understanding cloning vectors.
Cloning vectors are DNA molecules used to carry foreign DNA into host cells for replication and expression.
Step 2: Examples.
- Plasmids and bacteriophages are common bacterial cloning vectors.
- pBR322 is a widely used plasmid vector in bacteria.
- T-DNA, however, is a part of Agrobacterium’s Ti plasmid, used for gene transfer in plants, not bacteria.
Step 3: Conclusion.
Thus, T-DNA is not a cloning vector for bacteria.