Question

Which of the following is based on the principle of antigen-antibody interaction?

• A. PCR
• B. ELISA
• C. rDNA technology
• D. Gel Electrophoresis

Hint:

ELISA is a powerful tool in medical diagnostics for detecting diseases.

Answer 1

The Correct Option is B

The principle of antigen-antibody interaction is a fundamental concept in immunology and diagnostic techniques. Among the given options:

(A) PCR (Polymerase Chain Reaction) - Incorrect: PCR is based on DNA amplification using primers and DNA polymerase, not antigen-antibody interactions.

(B) ELISA (Enzyme-Linked Immunosorbent Assay) - Correct: ELISA directly utilizes the specific binding between antigens and antibodies for detection, often with an enzyme-linked system for visualization.

(C) rDNA technology - Incorrect: Recombinant DNA technology involves gene manipulation using restriction enzymes and ligases, unrelated to antigen-antibody interactions.

(D) Gel Electrophoresis - Incorrect: This technique separates molecules based on size and charge using an electric field, not immunological reactions.

The correct answer is (B) ELISA, as it is specifically designed to exploit the high specificity of antigen-antibody binding for diagnostic purposes.

Answer 2

The principle of antigen-antibody interaction is foundational to immunological techniques. In ELISA (Enzyme-Linked Immunosorbent Assay) , antibodies are used to detect specific antigens or vice versa. This interaction is visualized using enzymatic reactions that produce a color change.

Other options:

• (A): Incorrect because PCR amplifies DNA, not proteins or antigens.
• (B): Correct because ELISA relies on antigen-antibody interactions.
• (C): Incorrect because rDNA technology involves genetic engineering, not immune responses.
• (D): Incorrect because gel electrophoresis separates molecules based on size, not immune interactions.