Question

Which of the following enzymes is/are involved in the biogenesis of miRNA?

• A. Drosha
• B. Cas9
• C. XRCC4
• D. Dicer

Hint:

Drosha and Dicer are both RNase III enzymes that play critical roles in miRNA biogenesis. Drosha processes pri-miRNA in the nucleus, while Dicer processes pre-miRNA in the cytoplasm.

Answer 1

The Correct Option is A, D

Step 1: Understand the miRNA biogenesis process.
MicroRNAs (miRNAs) are small, non-coding RNA molecules involved in post-transcriptional regulation of gene expression. The biogenesis of miRNAs involves several key enzymes:
Step 2: Analyze the enzymes involved in miRNA biogenesis.
Drosha (A) is an RNase III enzyme that processes the primary miRNA (pri-miRNA) into precursor miRNA (pre-miRNA) in the nucleus. Drosha is a key enzyme in the early stages of miRNA biogenesis.
Cas9 (B) is involved in the CRISPR/Cas9 system, which is used for genome editing, not miRNA biogenesis.
XRCC4 (C) is involved in DNA repair and is not related to miRNA biogenesis.
Dicer (D) is another RNase III enzyme that processes the pre-miRNA into mature miRNA in the cytoplasm. Dicer plays a crucial role in the final steps of miRNA maturation.
Step 3: Conclusion.
The enzymes involved in the biogenesis of miRNA are Drosha and Dicer. Thus, the correct answer is \( \boxed{(A), (D)} \).