Step 1: Understand the concept of zygote formation.
In the context of human reproduction, brothers can develop from a single fertilized egg (zygote) that splits into two embryos. This process is known as monozygotic twinning or identical twins.
Step 2: Analyze the options.
- One zygote: If a single zygote divides into two embryos, identical brothers can develop.
- Two zygotes: This would result in fraternal twins, not identical brothers.
- Without zygote: Development without a zygote is not possible for human reproduction.
- Gamete: A gamete is an un-fertilized cell (sperm or egg) and cannot develop into a human alone.
Step 3: Conclusion.
The development of brothers from one zygote is the correct answer.
Final Answer:
\[
\boxed{One \; zygote}
\]