The dark reaction, also known as the Calvin cycle, does not require light directly but depends on the products of the light reactions. The dark reaction requires:
CO₂ (C): Acts as the substrate for carbon fixation.
ATP (D): Provides the energy needed for the reactions.
NADPH (E): Supplies the reducing power for the conversion of 3-phosphoglycerate into G3P.
Step 1: Light and chlorophyll are essential for the light reactions, but they are not directly required for the dark reactions.
Thus, the correct answer is (3) C, D, and E only.
The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :