Question

Which is the confirmatory test for HIV–AIDS?

• A. ELISA
• B. Western Blot
• C. CD4 Cell count
• D. RT-PCR

Hint:

Always follow a two-step testing strategy for HIV: screen with ELISA and confirm with Western Blot to ensure both sensitivity and specificity.

Answer 1

The Correct Option is B

The Western Blot test is considered a confirmatory diagnostic tool for HIV infection. While the ELISA test is typically used for initial screening due to its high sensitivity, it may yield false positives. Western Blot is used after a positive ELISA result to confirm the presence of specific HIV antibodies, offering higher specificity. It detects antibodies to individual HIV proteins, providing more conclusive evidence of infection.