Question

Which among the following is not a calibration parameter for UV visible spectrophotometer?
A. Control of wavelength 
B. Control of absorbances 
C. Resolution 
D. Control of transmitted radiation 
E. Spectral slit width 
Choose the correct answer from the options given below: 

• A. A, B and D only
• B. A, B, C and E only
• C. A, B and C only
• D. A and B only

Answer 1

The Correct Option is A

To find out which among the given options is not a calibration parameter for a UV visible spectrophotometer, we need to review the standard parameters for calibration in such instruments.

1. Control of wavelength: This is a crucial parameter in UV-Vis spectrophotometry as it ensures that the instrument is measuring the correct wavelength for the analysis of a sample. Hence, it is a calibration parameter.
2. Control of absorbances: This parameter checks if the instrument is accurately quantifying the absorbance of a sample, ensuring that the readings are correct. Therefore, it is a calibration parameter.
3. Resolution: This refers to the ability of the spectrophotometer to distinguish between closely spaced wavelengths. Although important for the performance of the instrument, it is not a direct calibration parameter in the traditional sense.
4. Control of transmitted radiation: This parameter is less relevant as a calibration parameter, as spectrophotometry is primarily concerned with absorbance, which is related to the amount of light absorbed rather than transmitted.
5. Spectral slit width: This parameter affects the bandwidth of light that passes through to the detector, impacting resolution and sensitivity. Calibration involving the spectral slit width helps ensure optimal performance; hence, it is a calibration parameter.

Based on the above evaluation, the parameters Control of wavelength, Control of absorbances, and Control of transmitted radiation are least consistent with traditional calibration requirements.

Conclusion: Therefore, the correct answer is A, B and D only. These three do not typically function as the calibration parameters for a UV visible spectrophotometer.