Question

What is the nature of mobile phase in normal phase chromatography?

• A. Polar
• B. Non-polar
• C. Mixture of polar and non-polar
• D. Can be polar and non-polar, it depends on the nature of analyte

Hint:

Normal phase: polar stationary + non-polar mobile phase.

Answer 1

The Correct Option is B

In normal phase chromatography, the stationary phase is polar (e.g., silica), and the mobile phase is non-polar (e.g., hexane). This setup separates analytes based on increasing polarity.