In the liquid state, a water molecule can form hydrogen bonds with surrounding water molecules due to its polar nature and the presence of two hydrogen atoms and two lone pairs of electrons on the oxygen atom. Each water molecule can accept two hydrogen bonds through its lone pairs and donate two hydrogen bonds through its hydrogen atoms. Therefore, the maximum number of hydrogen bonds a single water molecule can form is 4.
For hydrogen atom, $\lambda_1$ and $\lambda_2$ are the wavelengths corresponding to the transitions 1 and 2 respectively as shown in figure The ratio of $\lambda_1$ and $\lambda_2$ is $\frac{x}{32}$ The value of $x$ is
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 