Question

What is the main purpose of Polymerase Chain Reaction (PCR)?

• A. To sequence DNA
• B. To amplify specific DNA sequences
• C. To cut DNA into fragments
• D. To identify mutations in genes

Hint:

PCR is a powerful tool in molecular biology that helps amplify specific DNA regions for various applications, including cloning, gene expression analysis, and mutation detection.

Answer 1

The Correct Option is B

Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify specific DNA sequences. It allows researchers to make millions of copies of a particular DNA segment, making it easier to study in detail. The process involves repeated cycles of DNA denaturation, annealing of primers, and DNA synthesis. - Option (A) is incorrect because sequencing DNA is typically done through techniques like Sanger sequencing or next-generation sequencing, not PCR.
- Option (C) is incorrect because PCR does not cut DNA into fragments; it amplifies a specific segment of DNA.
- Option (D) is incorrect because PCR is not used specifically to identify mutations, although it can be used as a preliminary step in mutation detection techniques. Therefore, the correct answer is option (B).