To determine the acceleration due to gravity on the surface of a planet, we use the formula \( g = \frac{G \cdot M}{R^2} \), where \( G \) is the gravitational constant, \( M \) is the mass of the planet, and \( R \) is its radius. For Earth, the gravity \( g_{\text{Earth}} \) is given as 10 m/s². Let \( M_{\text{Earth}} \) and \( R_{\text{Earth}} \) be the mass and radius of Earth, respectively. On the new planet:
We substitute these into the formula for gravity: \( g = \frac{G \cdot (M_{\text{Earth}}/80)}{(\frac{R_{\text{Earth}}}{4})^2} = \frac{G \cdot M_{\text{Earth}}}{80 \cdot \frac{R_{\text{Earth}}^2}{16}} = \frac{16 \cdot G \cdot M_{\text{Earth}}}{80 \cdot R_{\text{Earth}}^2} = \frac{1}{5} \cdot \frac{G \cdot M_{\text{Earth}}}{R_{\text{Earth}}^2} = \frac{1}{5} \cdot g_{\text{Earth}} \). Since \( g_{\text{Earth}} = 10 \, \text{m/s}^2 \), it follows that \( g = \frac{1}{5} \times 10 = 2 \, \text{m/s}^2 \).

Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 