Upon exposure to UV radiation, DNA stained with ethidium bromide will show
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
Bright blue colour
Bright yellow colour
Bright orange colour
Bright red colour
The Correct Option is C
Solution and Explanation
When DNA is stained with ethidium bromide and exposed to ultraviolet (UV) radiation, it exhibits fluorescence due to the intercalation of the dye between the DNA base pairs. Ethidium bromide is a fluorescent molecule that absorbs UV light and re-emits it as visible light. The specific color emitted by ethidium bromide when bound to DNA and exposed to UV light is bright orange. This happens because ethidium bromide, upon absorbing UV light, enters an excited state and then returns to its ground state by emitting photons in the visible spectrum, specifically in the orange range, typically around 590 nm. Therefore, the correct answer is:
- Bright orange colour
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.