Question

Transfer of plasmids into animal cells can be achieved by

• A. Electroporation
• B. Liposome-mediated process
• C. Calcium chloride treatment
• D. Sucrose treatment

Answer 1

The Correct Option is A, B, C

The question pertains to methods of transferring plasmids into animal cells. This process is crucial in the field of genetic engineering and biotechnology to introduce new genes into cells, which can be used for research, therapy, or production of valuable molecules.

1. Electroporation: This technique uses an electric field to increase the permeability of the cell membrane. It allows plasmids to enter the cells by creating temporary pores in the cell membrane. Electroporation is widely used due to its efficiency in introducing DNA into cells without chemically altering the DNA.
2. Liposome-mediated method: Liposomes are small spherical vesicles that can encapsulate plasmid DNA. When liposomes fuse with the cell membrane, they deliver the DNA into the cell. This method is advantageous because it is less damaging to the cells compared to physical methods and can be used on a variety of cell types.
3. Calcium chloride treatment: Although more commonly associated with bacterial transformation, this method can also facilitate plasmid uptake in certain types of animal cells. The treatment induces competence in cells, enabling the entry of plasmid DNA by interaction with calcium ions, which may help stabilize DNA structures or alter the surface characteristics of the cell membrane.

In conclusion, all three methods mentioned — electroporation, liposome-mediated process, and calcium chloride treatment — are valid techniques for transferring plasmids into animal cells. Sucrose treatment is not typically used for this purpose, which makes it the odd one out in the provided options.