Question:
The role of DNA ligase in the construction of a recombinant DNA molecule is
The role of DNA ligase in the construction of a recombinant DNA molecule is
Updated On: Jul 5, 2022
- Formation of phosphodiester bond between two DNA fragments
- Formation of hydrogen bonds between sticky ends of DNA fragments
- Ligation of all purine and pyrimidine bases
- None of the above
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The Correct Option is A
Solution and Explanation
DNA ligases (joining or sealing enzymes) are also called genetic gum. They join two individual fragments of double stranded DNA by forming phosphodiester bonds between them. Thus, they help in sealing gaps in DNA fragments. Therefore, they act as a molecular glue. The enzyme used most often in the rDNA technology is $T_4$ DNA ligase.
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.