The relationship between the applied force \( F(X) \) (in Newton) on a body and its displacement \( X \) (in metre) is given below. The total amount of work done in moving the body from \( X = 0 \) to \( X = 4 \, \text{m} \) is ............ Joule. 
Step 1: Understanding the concept of work done.
The work done \( W \) by a variable force is given by the area under the force-displacement graph. The graph provided has three parts: a constant force from \( 0 \) to \( 2 \, \text{m} \), a linearly increasing force from \( 2 \) to \( 4 \, \text{m} \), and a constant force at \( X = 4 \).
Step 2: Calculating the area under the graph.
- From \( X = 0 \) to \( X = 2 \, \text{m} \), the force is constant at 2 N. The work done in this section is the area of a rectangle: \[ W_1 = \text{Force} \times \text{Displacement} = 2 \, \text{N} \times 2 \, \text{m} = 4 \, \text{J} \] - From \( X = 2 \) to \( X = 4 \, \text{m} \), the force increases linearly from 2 N to 4 N. The work done in this section is the area of a triangle: \[ W_2 = \frac{1}{2} \times \text{Base} \times \text{Height} = \frac{1}{2} \times 2 \, \text{m} \times (4 \, \text{N} - 2 \, \text{N}) = 2 \, \text{J} \]
Step 3: Total work done.
The total work done is the sum of the work in both sections: \[ W = W_1 + W_2 = 4 \, \text{J} + 2 \, \text{J} = 10 \, \text{J} \]
Step 4: Conclusion.
Thus, the total work done in moving the body from \( X = 0 \) to \( X = 4 \, \text{m} \) is \( 10 \, \text{J} \), and the correct answer is 10 Joules.

Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 