Question

The process in which sample DNA and probe DNA form double stranded DNA is called ______.

• A. electrophoresis
• B. restriction digestion
• C. hybridization
• D. amplification

Hint:

Hybridization is a key process in molecular biology where complementary DNA strands bind together to form a double helix.

Answer 1

The Correct Option is C

Step 1: Understanding hybridization.
Hybridization is the process where complementary strands of nucleic acids, such as DNA, bind together to form double-stranded molecules. In this case, probe DNA binds to sample DNA, forming double-stranded DNA.
Step 2: Analyzing the options.
(A) electrophoresis: Incorrect — Electrophoresis is a technique used to separate DNA fragments based on size, not for forming double-stranded DNA.
(B) restriction digestion: Incorrect — Restriction digestion involves cutting DNA into smaller fragments using enzymes, not forming double-stranded DNA.
(C) hybridization: Correct — Hybridization refers to the process of two complementary DNA strands binding to form a double-stranded DNA molecule.
(D) amplification: Incorrect — Amplification refers to the process of increasing the number of copies of a DNA segment (e.g., PCR), not forming double-stranded DNA.
Step 3: Conclusion.
The correct answer is (C) hybridization, as it describes the formation of double-stranded DNA from sample and probe DNA.