Question:

The phosphoester linkage in the nucleotides is between

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The 5' to 3' directionality in DNA/RNA strands is due to the phosphoester linkage between the 5' phosphate and 3' OH of adjacent nucleotides.
Updated On: Jun 18, 2025
  • phosphate group and OH of 3'C of a nucleotide
  • phosphate group and OH of 5'C of a nucleotide
  • phosphate group and H of 3'C of a nucleotide
  • phosphate group and H of 5'C of a nucleotide
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The Correct Option is B

Solution and Explanation

Step 1: In a nucleotide, the phosphoester linkage forms the backbone of DNA or RNA by connecting nucleotides.
Step 2: This linkage occurs between the phosphate group of one nucleotide and the hydroxyl (OH) group on the 5' carbon of the sugar in the next nucleotide.
Step 3: The bond is not formed with the 3' carbon’s OH in the backbone linkage, nor with a hydrogen atom.
Thus, the phosphoester linkage is between the phosphate group and the OH of 5'C of a nucleotide.
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