Question

The number of peptide fragments generated from the sequence: Ala-Trp-Val-Ala-Phe-Thr-Gly-Lys-Glu-Tyr-Asp-Ser-Lys on treatment with chymotrypsin is ..........

Hint:

Chymotrypsin cleaves peptides after aromatic amino acids, including phenylalanine, tyrosine, and tryptophan.

Answer 1

Correct Answer: 3.9

Step 1: Understand the specificity of chymotrypsin.
Chymotrypsin is a protease that cleaves peptide bonds on the carboxyl side of aromatic amino acids, such as phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp).

Step 2: Identify the cleavage sites.
In the given sequence, chymotrypsin will cleave after: - Phe (position 5) - Trp (position 2) - Tyr (position 9) The sequence contains 3 cleavage sites, resulting in 4 fragments.

Step 3: Conclusion.
The number of peptide fragments generated after treatment with chymotrypsin is 4.