Let $C$ be the circle $x^2 + (y - 1)^2 = 2$, $E_1$ and $E_2$ be two ellipses whose centres lie at the origin and major axes lie on the $x$-axis and $y$-axis respectively. Let the straight line $x + y = 3$ touch the curves $C$, $E_1$, and $E_2$ at $P(x_1, y_1)$, $Q(x_2, y_2)$, and $R(x_3, y_3)$ respectively. Given that $P$ is the mid-point of the line segment $QR$ and $PQ = \frac{2\sqrt{2}}{3}$, the value of $9(x_1 y_1 + x_2 y_2 + x_3 y_3)$ is equal to
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 