Question:
Tetracycline resistance gene of pBR322 consists of this restriction site:
Tetracycline resistance gene of pBR322 consists of this restriction site:
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pBR322 is a widely used cloning vector with Ampicillin and Tetracycline resistance genes.
Updated On: Mar 11, 2025
- BamHI
- EcoRI
- PstI
- HindIII
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Restriction Sites in pBR322
- The pBR322 plasmid contains two antibiotic resistance genes:
- Ampicillin resistance (bla gene).
- Tetracycline resistance gene.
- The BamHI restriction site is present in the tetracycline resistance gene, which allows for gene insertion.
- The pBR322 plasmid contains two antibiotic resistance genes:
- Ampicillin resistance (bla gene).
- Tetracycline resistance gene.
- The BamHI restriction site is present in the tetracycline resistance gene, which allows for gene insertion.
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