Question

Stationary phase for steroidal chromatography is:

• A. Acylated paper
• B. Carboxy Paper
• C. Kieselguhr paper
• D. Silica Paper

Hint:

Selecting the correct stationary phase depends on the polarity and nature of the analyte to ensure optimal separation.

Answer 1

The Correct Option is A

- In chromatography, the stationary phase is the phase that does not move and with which the compounds interact.
- Steroidal compounds are generally non-polar and require a stationary phase that can interact effectively to allow separation.
- Acylated paper is paper chemically modified with acyl groups, making it more hydrophobic and suitable for separating steroidal compounds due to better interaction with the non-polar steroids.
- Other options like carboxy paper, kieselguhr paper, and silica paper are typically used for different types of chromatography, such as polar compounds or different analytical purposes.
- Hence, acylated paper is the preferred stationary phase in steroidal chromatography to achieve efficient separation.