Question

Southern blotting is:

• (A) Attachment of probes to DNA fragments.
• (B) Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet.
• (C) Comparison of DNA fragments to two sources.
• (D) Transfer of DNA fragments to electrophoretic gel from cellulose membrane.

Answer 1

The Correct Option is A

Explanation:
Southern blotting is the attachment of probes to DNA fragments. Southern blotting is a laboratory technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membrane.Hence, the correct option is (A).