Retroviruses are commonly used in gene therapy and can be engineered to deliver approximately 8000 bases of single-stranded RNA. This RNA is reverse-transcribed into DNA, which integrates into the host genome, enabling stable expression of therapeutic genes.
(a) Write the palindromic nucleotide sequence for following sequence of DNA segment:
5' – GAATTC – 3'
(b) Name the restriction endonuclease that recognizes this sequence.
(c) How are sticky ends produced? Mention their role.