Question:

Restriction endonucleases

Updated On: May 19, 2024
  • are present in mammalian cells for degradation of DNA when the cell dies
  • are used in genetic engineering for ligating two DNA molecules
  • are used for in vitro DNA synthesis
  • are synthesized by bacteria as part of their defense mechanism.
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The Correct Option is D

Solution and Explanation

Restriction endonucleasses are enzymes that digest double stranded DNA following recognition of specific nucleotide sequences. This is achieved by cleaving the two phosphodiester bonds, one within each strand of the DNA duplex. They are found in bacteria and their function in bacteria is to cut up any invading virus as a part of its defense mechanism, thus restricting the multiplication of viruses in the bacterial cell. Different species of bacteria produce different restriction endonucleases.
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Concepts Used:

Biotechnology

Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."

Principles of Biotechnology:

Among many, the two core techniques that entitled the birth of modern biotechnology are :

  1. Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
  2. Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.