Question

Read the passage given below and answer the questions that follow: In recombinant DNA technology, restriction enzymes are used as they recognize and cut DNA within a specific recognition sequence. BamHI is one such restriction enzyme which binds at the recognition sequence 5’-G↓GATCC-3’ and cleaves this sequence between G and G on each strand, whereas AluI binds at the recognition sequence 5’-AG↓CT-3’ and cleaves these sequences between G and C on each strand.
If Alu I is used to cut the given DNA strand, how many DNA fragments would be formed? Write the sequence of each fragment formed with its polarity.
\begin{figure}[H] \centering \includegraphics[width=0.8\textwidth]{Screenshot 2025-01-17 115424.png} \caption{DNA sequence representation with complementary base pairing.} \label{fig:dna_sequence} \end{figure}

Hint:

Alu I is a restriction enzyme that recognizes specific palindromic sequences, cutting them to produce blunt ends.

Answer 1

The sequence contains three Alu I recognition sites. Fragments formed: 5’– CCGG–3’ 5’– ATCCTG–3’ 5’– CGAT–3’