Carboxylation is the fixation of $CO_{2}$ into a stable organic intermediate. It is the most crucial step of Calvin cycle where $CO_{2}$ is utilised for the carboxylation of $RuBP$ through the use of $ATP$ and $NADPH$ generated by the light reactions. This reaction is catalysed by enzyme $RuBisCO$ which results in the formation of two molecules of $3$-phosphoglyceric acid or $PGA$, which is the first stable product of photosynthesis.