Recombinant technology involves the manipulation of an organism's genetic material to introduce new genes, enabling the production of desired proteins or traits. Below is a simple diagram illustrating the process of recombinant DNA technology.
Step 1: Key Steps in Recombinant DNA Technology.
1. Gene Isolation: The desired gene is isolated from the organism's DNA using restriction enzymes.
2. Gene Insertion: The isolated gene is inserted into a vector (often a plasmid) using ligase enzymes.
3. Transformation: The recombinant DNA is introduced into a host organism (often bacteria) for replication.
4. Selection: The transformed cells are selected, usually using an antibiotic resistance marker.
5. Expression: The host organism expresses the inserted gene, producing the desired protein.
Final Answer:
The diagram above illustrates the key steps in recombinant DNA technology, including gene isolation, insertion into a vector, transformation, selection, and protein expression.
