We need to calculate the force on the middle charge at \(x = 2 \, \text{nm}\) due to the other two charges at \(x = 0\) and \(x = 6 \, \text{nm}\). According to Coulomb's Law, the force between two point charges \(q_1\) and \(q_2\) separated by a distance \(r\) is given by:
\(F = \frac{k \cdot |q_1 \cdot q_2|}{r^2}\)
Here, the force between charges separated by 2 nm is 2 pN. We need to find:
- The distance between \(x = 0\) and \(x = 2\) is 2 nm.
- The distance between \(x = 2\) and \(x = 6\) is 4 nm.
1. Since the force \(F = 2 \, \text{pN}\) for 2 nm separation, for \(F_{01}\) (same distance), the force is 2 pN.
2. For \(F_{21}\), at 4 nm, using inverse square law:
\(F_{21} = 2 \, \text{pN} \times \left(\frac{2}{4}\right)^2 = 2 \, \text{pN} \times \frac{1}{4} = 0.5 \, \text{pN}\)
The force directions for \(F_{01}\) and \(F_{21}\) are opposite (assuming positive direction is towards larger x). Therefore, net force \(F_{\text{net}}\) on middle charge:
\(F_{\text{net}} = F_{01} - F_{21} = 2 \, \text{pN} - 0.5 \, \text{pN} = 1.5 \, \text{pN}\)
This calculated net force = \(1.5 \, \text{pN}\)
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 