| Group I | Group II |
| P) Treponema pallidum | 1) Sleeping sickness |
| Q) Trypanosoma cruzi | 2) Whooping cough |
| R) Trypanosoma gambiense | 3) Chagas disease |
| S) Bordetella pertussis | 4) Syphilis |
To solve this question, we need to correctly match the microorganisms listed in Group I with the diseases listed in Group II.
Based on the above analysis, the correct matches are:
| Microorganism (Group I) | Human Disease (Group II) |
| P) Treponema pallidum | 4) Syphilis |
| Q) Trypanosoma cruzi | 3) Chagas disease |
| R) Trypanosoma gambiense | 1) Sleeping sickness |
| S) Bordetella pertussis | 2) Whooping cough |
Therefore, the correct answer is: P-4, Q-3, R-1, S-2.
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 