Question

Match the following:

• A. A-III, B-IV, C-I, D-II
• B. A-III, B-IV, C-II, D-I
• C. A-III, B-I, C-II, D-IV
• D. A-III, B-I, C-II, D-IV

Hint:

Understanding the roles of these molecular biology tools is essential for applications in genetic engineering and biotechnology.

Answer 1

The Correct Option is B

- A) pBR322 is a plasmid used in genetic engineering involving E. Coli (III).
- B) T-DNA is a segment from the Ti plasmid of Agrobacterium tumefaciens (IV), used in plant genetic engineering.
- C) COSMID is a type of plasmid engineered to include a bacteriophage lambda cos site, which allows it to be packaged into lambda phage particles for bacterial infection, making it a plasmid with ‘cos’ site (II).
- D) DNA Ligase is an enzyme crucial for DNA replication and repair, primarily used to join DNA fragments (I) by catalyzing the formation of phosphodiester bonds. Thus, the correct matches according to the statements and options provided are:
- A) pBR322 → III
- B) T-DNA → IV
C) COSMID → II
D) DNA Ligase → I