Match the diseases in Group I with their causative organisms in Group II
Group I Group II
(P) Syphilis (1) Shigella dysenteriae
(Q) Bacillary dysentery (2) Bordetella pertussis
(R) Gas gangrene (3) Treponema pallidum
(S) Whooping cough (4) Clostridium perfringens
Step 1: Understanding the causative organisms.
- Syphilis is caused by Treponema pallidum (P-3).
- Bacillary dysentery is caused by Shigella dysenteriae (Q-1).
- Gas gangrene is caused by Clostridium perfringens (R-4).
- Whooping cough is caused by Bordetella pertussis (S-2).
Step 2: Analyzing the options.
(A) P-2, Q-1, R-4, S-3: Incorrect — Syphilis is caused by Treponema pallidum, not Shigella.
(B) P-4, Q-3, R-1, S-2: Incorrect — The pairing of syphilis with Clostridium is incorrect.
(C) P-2, Q-3, R-1, S-4: Incorrect — The pairings are incorrect.
(D) P-3, Q-1, R-4, S-2: Correct — This correctly matches the diseases with their causative organisms.
Step 3: Conclusion.
The correct answer is (D) P-3, Q-1, R-4, S-2.
Read the following passage and answer the questions that follow. Prevention is the frontline response to drug use. Effective interventions address the underlying conditions contributing to drug use, such as a lack of connection to family or community, instability, insecurity, trauma, mental health issues, etc. When addressed, these factors can effectively prevent the initiation of drug use and the progression to drug use disorders. Study the few key figures of drug use given below and answer the questions that follow. 
(a) What do you infer from the figures in Table No. 1 about the people with drug use disorders, 2022 (in million)? State any two of your observations.
(b) How are Hepatitis C and HIV related to drug use disorders by people, as shown in Table No. 2? State the correlation between the two.
(c)
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 