List I | List II |
---|---|
(A) Radiation hybrid mapping | (I) DNA can be cut into large fragments and circularized for use in chromosome walking |
(B) Sequence tagged site (STS) mapping | (II) Useful for cloning of overlapping DNA fragments (restricted to about 200 kb) |
(C) Chromosome jumping | (III) Fragment genome into large pieces and locate markers and genes |
(D) Chromosome walking | (IV) Applicable to any part of DNA sequence if some sequence information is available |
- Radiation hybrid mapping involves allows researchers to skip over regions of DNA to locate distant markers and genes.
- Sequence tagged site (STS) mapping is used to study DNA regions when sequence information is available.
- Chromosome jumping breaking DNA into large fragments and circularizing them for chromosome walking.
- Chromosome walking is essential for overlapping DNA fragment cloning.