Manipulation of DNA in genetic engineering became possible due to the discovery of
The correct option is (A) : Restriction endonuclease
The discovery of restriction endonuclease made it feasible to manipulate DNA for use in genetic engineering. There are several hundred restriction enzymes that have been isolated from bacterial cells and are used by molecular biologists as tools. Each one has a unique sequence requirement that determines where it will cut DNA. As a result, when a restriction enzyme breaks down DNA, it produces a distinctive collection of fragments that can be separated by electrophoresis and then examined.
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out
In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
List - I | List – II | ||
A. | Residual Volume | i. | Maximum volume of air that can be breathed in after forced expiration |
B. | Vital Capacity | ii. | Volume of air inspired or expired during normal respiration |
C. | Expiratory Capacity | iii. | Volume of air remaining in lungs after forcible expiration |
D. | Tidal Volume | iv. | Total volume of air expired after normal inspiration |
List I | List II | ||
---|---|---|---|
A | Robert May | I | Species-Area relationship |
B | Alexander von Humboldt | II | Long term ecosystem experiment using out door plots |
C | Paul Ehrlich | III | Global species diversity at about 7 million |
D | David Tilman | IV | Rivet popper hypothesis |
Biotechnology is the technology that utilizes biological systems, living organisms, or parts of this to develop or create different products.
For example, brewing and baking bread fall within the concept of biotechnology (use of yeast (= living organism) to produce the desired product).
Alternative to conventional farming: A possible solution is the use of genetically modified crops which is an alternative path to conventional farming.
In the area of healthcare, recombinant DNA technological processes have made an immense impact which enables the mass production of safe and more effective therapeutic drugs.
A collection of methods that allows the correction of a gene defect that has been diagnosed in a child/embryo is called Gene Therapy.
For editing genomes, CRISPR is a simple yet powerful tool which allows researchers to easily alter DNA sequences and modify gene function.