Question:

Manipulation of DNA in genetic engineering became possible due to the discovery of

Updated On: May 4, 2024

Restriction endonuclease
DNA ligase
Transcriptase
Primase

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The Correct Option is A

Solution and Explanation

The correct option is (A) : Restriction endonuclease

The discovery of restriction endonuclease made it feasible to manipulate DNA for use in genetic engineering. There are several hundred restriction enzymes that have been isolated from bacterial cells and are used by molecular biologists as tools. Each one has a unique sequence requirement that determines where it will cut DNA. As a result, when a restriction enzyme breaks down DNA, it produces a distinctive collection of fragments that can be separated by electrophoresis and then examined.

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Questions Asked in NEET exam

Match List I with List II :

List - I		List – II
A.	Residual Volume	i.	Maximum volume of air that can be breathed in after forced expiration
B.	Vital Capacity	ii.	Volume of air inspired or expired during normal respiration
C.	Expiratory Capacity	iii.	Volume of air remaining in lungs after forcible expiration
D.	Tidal Volume	iv.	Total volume of air expired after normal inspiration

Choose the correct answer from the options given below :

NEET (UG) - 2024
The respiratory system

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Match List I with List II:

List I		List II
A	Robert May	I	Species-Area relationship
B	Alexander von Humboldt	II	Long term ecosystem experiment using out door plots
C	Paul Ehrlich	III	Global species diversity at about 7 million
D	David Tilman	IV	Rivet popper hypothesis

Choose the correct answer from the options given below:

NEET (UG) - 2024
Species diversity

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Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A : The potential (V) at any axial point, at 2 m distance(r) from the centre of the dipole of dipole moment vector \(\vec{P}\) of magnitude, 4 × 10^-6 C m, is ± 9 × 10³ V.
(Take \(\frac{1}{4\pi\epsilon_0}=9\times10^9\) SI units)
Reason R : \(V=±\frac{2P}{4\pi \epsilon_0r^2}\), where r is the distance of any axial point, situated at 2 m from the centre of the dipole.
In the light of the above statements, choose the correct answer from the options given below :
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Concepts Used:

Applications of Biotechnology

Biotechnology is the technology that utilizes biological systems, living organisms, or parts of this to develop or create different products.

For example, brewing and baking bread fall within the concept of biotechnology (use of yeast (= living organism) to produce the desired product).

Agriculture

Alternative to conventional farming: A possible solution is the use of genetically modified crops which is an alternative path to conventional farming.

Medicine

In the area of healthcare, recombinant DNA technological processes have made an immense impact which enables the mass production of safe and more effective therapeutic drugs.

Gene Therapy

A collection of methods that allows the correction of a gene defect that has been diagnosed in a child/embryo is called Gene Therapy.

CRISPR technology

For editing genomes, CRISPR is a simple yet powerful tool which allows researchers to easily alter DNA sequences and modify gene function.