Question

(iv) Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?

Hint:

Insertional inactivation is a biosafe and effective method for identifying recombinant DNA.

Answer 1

Insertional inactivation involves disrupting a functional gene by inserting foreign DNA, providing a clear visual distinction between recombinant and non-recombinant colonies. Advantages include: 1. Biosafety: Avoids the use of antibiotic resistance genes, reducing environmental risks. 2. Simpler identification: Recombinant colonies can be visually identified (e.g., by loss of color). 3. Cost-effectiveness: Reduces reliance on antibiotics and additional screening steps. This method is efficient and environmentally friendly, making it a preferred choice in modern cloning practices. % Quick tip